PrimerPlex v2.73
Oligo design for multiplex PCR and high throughput SNP genotyping and analysis.
PrimerPlex Editor's review
The multiplex assays are used for gene expression analysis (end point detection using gel electrophoresis) and for high throughput SNP applications such as genotyping, pathogen detection, strain typing, and haplotyping. To ensure specificity, the oligos are designed after avoiding the regions of homologies identified by a BLAST search. PrimerPlex then checks the oligos for cross reactivity and minimizes Tm mismatches to give you the best possible multiplex set. In the process, it analyzes millions of possible multiplex primer sets in a few seconds and presents a list of alternate sets to you as well.What's new in this version:The latest changes to the genomic databases at the NCBI-BLAST along with fixes to reported problems are accommodated.
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